Journal: Antioxidants

Article Title: Combinatorial Effects of the Natural Products Arctigenin, Chlorogenic Acid, and Cinnamaldehyde Commit Oxidation Assassination on Breast Cancer Cells

doi: 10.3390/antiox11030591

Figure Lengend Snippet: Flow cytometry channel voltages in each cell line.

Article Snippet: HCC1419 (CRL-2326 from ATCC, Manassas, VA, USA) cells were seeded at 10,000 cells/well and treated for 6 days.

Techniques: Flow Cytometry

Journal: Antioxidants

Article Title: Combinatorial Effects of the Natural Products Arctigenin, Chlorogenic Acid, and Cinnamaldehyde Commit Oxidation Assassination on Breast Cancer Cells

doi: 10.3390/antiox11030591

Figure Lengend Snippet: IC 50 concentrations of CA, CGA, and Arc-treated breast cancer cells over time. Cell growth was determined by counting the number of cells in each group on each day. IC 50 concentrations of ( A ) CA-treated MCF-7 cells, ( B ) CGA-treated MCF-7 cells, ( C ) Arc-treated MCF-7 cells, ( D ) CA-treated MDA-MB-231 cells, ( E ) CGA-treated MDA-MB-231 cells, ( F ) Arc-treated MDA-MB-231 cells, ( G ) CA-treated HCC1419 cells, ( H ) CGA-treated HCC1419 cells, and ( I ) Arc-treated HCC1419 cells. Each bar represents the mean ± SEM of three biological replicates ( n = 3).

Article Snippet: HCC1419 (CRL-2326 from ATCC, Manassas, VA, USA) cells were seeded at 10,000 cells/well and treated for 6 days.

Techniques:

Journal: Antioxidants

Article Title: Combinatorial Effects of the Natural Products Arctigenin, Chlorogenic Acid, and Cinnamaldehyde Commit Oxidation Assassination on Breast Cancer Cells

doi: 10.3390/antiox11030591

Figure Lengend Snippet: Breast cancer cell growth curves treated with combinations of CA, CGA, and Arc in ( A ) MCF-7, ( B ) MDA-MB-231, and ( C ) HCC1419 cells. Cell growth was determined by counting the number of cells in each group on each day for 6/7 days. Each bar represents the mean ± SEM of three biological replicates ( n = 3). Groups were compared using a one-way ANOVA. The results of the statistical analysis are summarized in . Cell growth differences between groups were considered significant at p ≤ 0.05.

Article Snippet: HCC1419 (CRL-2326 from ATCC, Manassas, VA, USA) cells were seeded at 10,000 cells/well and treated for 6 days.

Techniques:

Journal: Antioxidants

Article Title: Combinatorial Effects of the Natural Products Arctigenin, Chlorogenic Acid, and Cinnamaldehyde Commit Oxidation Assassination on Breast Cancer Cells

doi: 10.3390/antiox11030591

Figure Lengend Snippet: Ratios of cell numbers of each treatment group to the untreated group in ( A ) MC7-7, ( B ) MDA-MB-231, and ( C ) HCC1419 breast cancer cells. Each bar represents the mean ± SEM of three biological replicates ( n = 3). The treatment groups were compared using a one-way ANOVA. The results of the statistical analysis are summarized in . Cell growth differences between treatment groups were considered significant at p ≤ 0.05.

Article Snippet: HCC1419 (CRL-2326 from ATCC, Manassas, VA, USA) cells were seeded at 10,000 cells/well and treated for 6 days.

Techniques:

Journal: Antioxidants

Article Title: Combinatorial Effects of the Natural Products Arctigenin, Chlorogenic Acid, and Cinnamaldehyde Commit Oxidation Assassination on Breast Cancer Cells

doi: 10.3390/antiox11030591

Figure Lengend Snippet: Cell death and live cell percentages in ( A ) MCF-7, ( B ) MDA-MB-231, and ( C ) HCC1419 breast cancer cells treated with CA, CGA, and Arc, alone or in combination, for 96 h. Green bars represent cells that are ΔΨm-positive and annexin V-negative (live cells); brown bars denote cells that are ΔΨm-positive and annexin V-positive (early cell death); red bars show cells that are ΔΨm-negative and annexin V-positive (late cell death); and black bars represent cells that are ΔΨm-negative and annexin V-negative (late cell death). Each green bar in the % live cell graphs represents the mean of means ± SEM from 3 biological and experimental replicates ( n = 3) showing the percentage of live cells at 96 h. Twenty-four-hour 10 µM Sutent treatment was used as a positive control for Annexin V-FITC and 30 min 0.1% Triton-X treatment was used as a double-negative staining control. The groups were compared using a one-way ANOVA. Differences in the percentage of live cells between groups were considered significant at p ≤ 0.05. Statistical significance compared to untreated: * = p < 0.05; ** = p < 0.01; *** = p < 0.001.

Article Snippet: HCC1419 (CRL-2326 from ATCC, Manassas, VA, USA) cells were seeded at 10,000 cells/well and treated for 6 days.

Techniques: Positive Control, Negative Staining, Control